Interleukin-10 gene transfer to peritoneal mesothelial cells suppresses peritoneal dissemination of gastric cancer cells due to a persistently high concentration in the peritoneal cavity.

{"article_title":"Liberation From Mechanical Ventilation in Critically Ill Adults: Executive Summary of an Official American College of Chest Physicians\/American Thoracic Society Clinical Practice Guideline.","author":"Schmidt GA, Girard TD, Kress JP, Morris PE, Ouellette DR, Alhazzani W, Burns SM, Epstein SK, Esteban A, Fan E, Ferrer M, Fraser GL, Gong MN, Hough CL, Mehta S, Nanchal R, Patel S, Pawlik AJ, Schweickert WD, Sessler CN, Str\u00f8m T, Wilson KC, Truwit JD","journal_title":"Chest","issn":"1931-3543","isbn":"","publication_date":"2008-01-01","volume":"15","issue":"1","first_page":"160","page_count":"","accession_number":"27818329","doi":"","publisher":"Elsevier","doctype":"Journal Article","subjects":"Critical Illness therapy; Respiration, Artificial methods; Adult; Aged; Clinical Decision-Making; Critical Care methods; Critical Care standards; Evidence-Based Emergency Medicine methods; Humans; United States","interest_area":["Emergency Medicine"," Cardiology"," Pulmonology"],"abstract":"Background: This clinical practice guideline addresses six questions related to liberation from mechanical ventilation in critically ill adults. It is the result of a collaborative effort between the American Thoracic Society (ATS) and the American College of Chest Physicians (CHEST). Methods: A multidisciplinary panel posed six clinical questions in a population, intervention, comparator, outcomes (PICO) format. A comprehensive literature search and evidence synthesis was performed for each question, which included appraising the quality of evidence using the Grading of Recommendations, Assessment, Development, and Evaluation (GRADE) approach. The Evidence-to-Decision framework was applied to each question, requiring the panel to evaluate and weigh the importance of the problem, confidence in the evidence, certainty about how much the public values the main outcomes, magnitude and balance of desirable and undesirable outcomes, resources and costs associated with the intervention, impact on health disparities, and acceptability and feasibility of the intervention. Results: Evidence-based recommendations were formulated and graded initially by subcommittees and then modified following full panel discussions. The recommendations were confirmed by confidential electronic voting; approval required that at least 80% of the panel members agree with the recommendation. Conclusions: The panel provides recommendations regarding liberation from mechanical ventilation. The details regarding the evidence and rationale for each recommendation are presented in the American Journal of Respiratory and Critical Care Medicine and CHEST. Copyright \u00a9 2016 American College of Chest Physicians. Published by Elsevier Inc. All rights reserved.","url":"https:\/\/search.ebscohost.com\/login.aspx?direct=true&db=mdl&AN=27818329","isPdfLink":true,"isSAML":false,"an":"27818329","number_other":"","type_pub":"","issn_electronic":"1931-3543","languages":"English","language":"eng","date_entry":"","date_update":"","titleSource":"Cancer Gene Therapy. Jan2008, Vol. 15 Issue 1, p51-59. 9p. 2 Color Photographs, 4 Graphs.","date_pub_cy":"","type_document":"","contract_publisher":"","authored_on":"2008-01-01","description":"Interleukin (IL)-10 has potent biological properties including an inhibitory action on the proliferation and metastasis of various cancer cells. However, it is difficult to maintain a high concentration of this cytokine as it has a short half life. In this study, we evaluated whether peritoneal mesothelial cells (PMCs) could be suitable for maintaining a high concentration of IL-10 using adenoviral gene transfer. We also evaluated the therapeutic effects of an intraperitoneal injection with adenoviral vector containing mouse IL-10 gene (Ad-mIL-10) using a mouse peritoneal dissemination model of MKN45 gastric cancer cells. We demonstrated that in vitro transfection efficiency of a recombinant adenovirus containing the bacterial \u03b2-galactosidase gene (Ad-LacZ) was approximately 10-fold higher for primarily isolated PMCs than MKN45. The entire peritoneum was transfected until 3 weeks after an intraperitoneal Ad-LacZ injection. Ad-mIL-10 treatment increased intraperitoneal IL-10 levels until 3 weeks after treatment, and then significantly inhibited peritoneal cancer growth by inhibiting angiogenesis. This treatment also improved cachexia and prolonged mice survival. We thus concluded that IL-10 gene transfer in PMCs could be a new strategy for the prevention of peritoneal dissemination of gastric cancer due to the resulting persistently high IL-10 concentration in the peritoneal cavity.Cancer Gene Therapy (2008) 15, 51\u201359; doi:10.1038\/sj.cgt.7701104; published online 9 November 2007 [ABSTRACT FROM AUTHOR]","upload_link":"https:\/\/search.ebscohost.com\/login.aspx?direct=true&site=eds-live&db=cxh&AN=27818329&authtype=shib&custid=ns346513&group=main&profile=eds","no_of_pages":"","authored_by":"Tanaka, F., Tominaga, K., Shiota, M., Ochi, M., Kuwamura, H., Tanigawa, T., Watanabe, T., Fujiwara, Y., Oshitani, N., Higuchi, K., Iwao, H., Arakawa, T.","header":{"DbId":"cxh","DbLabel":"Biomedical Reference Collection: Corporate","An":"27818329","RelevancyScore":"676","PubType":"Academic Journal","PubTypeId":"academicJournal","PreciseRelevancyScore":"675.905517578125"},"plink":"https:\/\/search.ebscohost.com\/login.aspx?direct=true&site=eds-live&db=cxh&AN=27818329&authtype=shib&custid=ns346513&group=main&profile=eds","issn_print":"09291903","physicalDescription":{"Pagination":{"PageCount":"9","StartPage":"51"}},"additionalInfo":{"Authored_By":"Tanaka, F., Tominaga, K., Shiota, M., Ochi, M., Kuwamura, H., Tanigawa, T., Watanabe, T., Fujiwara, Y., Oshitani, N., Higuchi, K., Iwao, H., Arakawa, T.","Published_Date":"2008-01-01","Source":"Cancer Gene Therapy. Jan2008, Vol. 15 Issue 1, p51-59. 9p. 2 Color Photographs, 4 Graphs.","Languages":"English","Subjects":"INTERLEUKIN-10, GENETIC transformation, GENETIC recombination, CANCER cells, MICROBIAL genetics","Title_Abbreviations":"Cancer Gene Therapy","Volume":"15"}}