Event Data
{"title":"Breast Imaging","subtitle":"A Core Review","e_issn":null,"url":"https:\/\/search.ebscohost.com\/login.aspx?direct=true&db=e600tww&AN=1483674&site=ehost-live&scope=site","series":"Core Review","edition":"Second edition","author":"Biren A. Shah-Sabala Mandava","contract_publisher":"","imprint_publisher":"Wolters Kluwer Health","pub_year":"2018","interest_area":["Oncology"," Obstetrics & Gynecology"," Radiology"],"lcc":"RG493.5.R33 B74 2018eb","lcsh":"Breast--Radiography--Methods.","language":"eng","format":"EPUB","downloadable":"TRUE","fiction":"FALSE","abridged":"","book_id":"1922093","isbn":"9781496358202","eisbn":"9781496358226","ocn":"1059514093","ddc":"618.1\/907572","isPdfLink":false,"isSAML":true,"an":"1483674","number_other":"","type_pub":"","isbn_print":"9781681080246","isbn_electronic":"9781681080239","languages":"English","date_entry":"","date_update":"","doi":"","titleSource":"","date_pub_cy":"","type_document":"","volume":null,"issue":null,"authored_on":"2017-01-01","description":"Advances in Modern Medicine introduces recent advanced medical practices performed at the Kure Medical Center and Chugoku Cancer Center (KMCCCC) - one of the leading hospitals in Japan - to those working in the field of medicine throughout the world, including physicians, surgeons, pharmacists, psychologists, medical engineers, medical technologists, nurses, and students. Readers will be updated on the general trends in modern medicine relevant to a variety of medical specialties performed at KMCCCC. The volume covers topics such as cancer management, acute phase reaction against a national-level disaster, depression management, emergency medicine, hepatobiliary and gastrointestinal diseases, orthopedics, organ transportation, infection control, blood disease, chronic kidney disease, palliative care, dermatology, ophthalmology, pathology, and nursing for cancer patients. Aspiring medical students can learn more about the latest developments in their field of interest, while patients can learn about treatment options available for different diseases.","publication_date":"2017-01-01","upload_link":"https:\/\/dx.doi.org\/doi:10.1210\/mend-5-6-741","no_of_pages":"","authored_by":"Wataru Kamiike, Kiyomi Taniyama, Wataru Kamiike, Kiyomi Taniyama","header":{"DbId":"mdl","DbLabel":"MEDLINE Ultimate","An":"1922093","RelevancyScore":"680","PubType":"Academic Journal","PubTypeId":"academicJournal","PreciseRelevancyScore":"680.100769042969"},"plink":"https:\/\/search.ebscohost.com\/login.aspx?direct=true&site=eds-live&db=mdl&AN=1922093&authtype=shib&custid=ns346513&group=main&profile=eds","additionalInfo":{"Authored_By":"Bonewald LF, Wakefield L, Oreffo RO, Escobedo A, Twardzik DR, Mundy GR","Subjects":"Animals, Blood Platelets drug effects, Blood Platelets physiology, Blotting, Western, Bone and Bones cytology, Cell Division drug effects, Cell Line, Cells, Cultured, Chromatography, Gel, Chromatography, High Pressure Liquid, Chromatography, Ion Exchange, DNA Replication drug effects, Electrophoresis, Polyacrylamide Gel, Humans, Mice, Mice, Inbred ICR, Molecular Weight, Protein Precursors genetics, Recombinant Proteins isolation & purification, Recombinant Proteins pharmacology, Transfection, Transforming Growth Factor beta genetics, Transforming Growth Factor beta pharmacology, Bone and Bones physiology, Protein Precursors isolation & purification, Transforming Growth Factor beta isolation & purification","Languages":"English","Published_Date":"1991-06-01","Title_Abbreviations":"Molecular endocrinology (Baltimore, Md.)","Volume":"5"}}
Additional Info
["Bonewald LF, Wakefield L, Oreffo RO, Escobedo A, Twardzik DR, Mundy GR","Animals, Blood Platelets drug effects, Blood Platelets physiology, Blotting, Western, Bone and Bones cytology, Cell Division drug effects, Cell Line, Cells, Cultured, Chromatography, Gel, Chromatography, High Pressure Liquid, Chromatography, Ion Exchange, DNA Replication drug effects, Electrophoresis, Polyacrylamide Gel, Humans, Mice, Mice, Inbred ICR, Molecular Weight, Protein Precursors genetics, Recombinant Proteins isolation & purification, Recombinant Proteins pharmacology, Transfection, Transforming Growth Factor beta genetics, Transforming Growth Factor beta pharmacology, Bone and Bones physiology, Protein Precursors isolation & purification, Transforming Growth Factor beta isolation & purification","English","1991-06-01","Molecular endocrinology (Baltimore, Md.)","5"]
Description
Transforming growth factor-beta (TGF beta) is produced by most tissues, including bone, as a complex that is biologically inert. Release of TGF beta homodimer from this latent complex is necessary for TGF beta to exert effects on target cells. Thus, the nature of the latent complex and the mechanisms responsible for TGF beta release are the key to understanding TGF beta actions. We have found that murine calvarial bone cultures secrete multiple latent forms of TGF beta. Using analytical chromatography and Western blot analysis, we have compared bone latent TGF beta with the previously characterized latent complex present in platelets and with simian TGF beta precursor, which is stably expressed in a latent form by Chinese hamster ovarian (CHO) cells. A major component of the bone material appears to be a latent complex of 100 kDa, consisting of mature TGF beta (25-kDa homodimer). Like the recombinant TGF beta precursor, it elutes from a Mono-Q fast pressure liquid chromatography anion exchange column at 0.2 M NaCl and shows a very similar banding pattern on Western blots. Thus, this bone complex closely resembles recombinant TGF beta precursor expressed in a latent form by CHO cells and differs from the naturally occurring platelet complex, which has an additional 135-kDa binding protein that is bound through disulfide bonds to the precursor proregion. Western blot analysis also indicates that, like CHO cells, which express recombinant TGF beta precursor, but unlike other cell types, the bone cultures secrete detectable amounts of uncleaved TGF beta precursor. The bone calvarial culture is the first example of a naturally occurring system that expresses the 100-kDa latent TGF beta complex.
